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Biotechnology / Escherichia coli / Cell lines / Shiga toxin / Enterobacteria / Escherichia coli O157:H7 / ELISA / Cell culture / Recombinant DNA / Biology / Bacteria / AB5 toxins


Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle
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Frickenhausen / Stx1 / Nidderau / Hilden / Freiburg / Taufkirchen / Leipzig / Giessen / Suffolk / Heidelberg / Thornhill / Amsterdam / Puchheim / Wiesbaden / Gaithersburg / Darmstadt / Wesel / Hamburg / Chicago / /

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SPSS Inc. / IgG / American Fluoroseal Corp. / HISS Diagnostics / Merck KGaA / Sigma-Aldrich Chemie GmbH / Qiagen / Pearson / Serva Electrophoresis GmbH / LGC-Promochem GmbH / Creative Commons / Dianova GmbH / Jackson ImmunoResearch Europe Ltd. / /

Country

Germany / Netherlands / United States / Canada / United Kingdom / /

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Facility

Institute of Hygiene / Justus Liebig University / Greenhouse Geisser Test / Royal Veterinary College / Institute of Molecular Pathogenesis / carried out applying Greenhouse Geisser Test / /

IndustryTerm

test systems / food chain / food-borne pathogens / rStxWT stock solutions / in vivo applications / stock solutions / /

MedicalCondition

infections / oedema disease / experimental STEC infection / experimental infection / human infections / hemorrhagic colitis / diseases / EHEC infection / STEC infection / infectious diseases / infection / STEC infections / human disease / hemolytic-uremic syndrome / significantly delayed following experimental infection / /

MedicalTreatment

Immunization / vaccination / vaccinations / /

Organization

Justus Liebig University / Institute of Hygiene and Infectious Diseases of Animals / Royal Veterinary College / London / Institute of Molecular Pathogenesis / Friedrich-Loeffler-Institut / /

Person

Gene Relevant / Dirk Werling / /

Position

author / representative / reporter / /

Product

QuickChange / Penicillin / Gentamicin / Amphotericin B / /

ProvinceOrState

Illinois / Ontario / /

Technology

antibodies / directed mutagenesis / /

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