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City La Jolla / / Company Qiagen / Merck / BD Biosciences / / Country United States / / Currency cent / / / Event FDA Phase / / Facility The Salk Institute / Howard Hughes Medical Institute / University of California / / IndustryTerm reverse tet transcriptional control systems / restriction site / optical imaging / / MedicalCondition Leukaemia disease / double infection / infection / Burkitt lymphoma cell line Ramos / / MedicalTreatment antibiotics / AID / / Organization Tsien2 1The Jack H. Skirball Center for Chemical Biology and Proteomics / Salk Institute for Biological Studies / US Department of Energy / Damon Runyon Cancer Research Foundation / Howard Hughes Medical Institute / University of California at San Diego / / Person J. Gene Med / Nat / / Position rt / representative / Harper / / Product Balanced Salt / sodium bicarbonate / doxycycline / Dimethyl sulfoxide / RetroMax / / ProvinceOrState California / / PublishedMedium NATURE PROTOCOLS / / Technology Proteomics / inverse PCR protocol / electroporation / directed evolution / antibodies / gene expression / NATURE PROTOCOLS / cloning / 1347 PROTOCOL / recombination / gel electrophoresis / / URL http / SocialTag 
PROTOCOL Evolving proteins in mammalian cells using somatic hypermutation Lei Wang1 & Roger Y Tsien2 1The Jack H. Skirball Center for Chemical Biology and Proteomics,