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Fluorescence / Fluorescent dyes / Calcium / Calcium signaling / Cell biology / Förster resonance energy transfer / Cameleon / Yellow fluorescent protein / Green fluorescent protein / Biology / Chemistry / Biochemistry


PROTOCOL Measuring calcium signaling using genetically targetable fluorescent indicators Amy E Palmer1 & Roger Y Tsien2 1Department of Chemistry and
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Document Date: 2014-07-01 03:40:01


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Other CaM / a FRET / Shaner / New York / D3cpv / /

Company

Nikon / Roche Diagnostics / Olympus / Fisher Scientific / MatTek Corporation / Qiagen / Dow Corning Corporation / Chroma Technology Corp. / HBO / Palmer A.E. / Cold Spring Harbor Laboratory Press / Microsoft / Corning / Roper Scientific Inc. / /

Country

United States / /

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Event

FDA Phase / /

Facility

Howard Hughes Medical Institute / ER store / Plant Physiol / CaMP CaM / University of California / University of Colorado / /

IndustryTerm

efficiency imaging / experimental systems / stock solution / stable cell line using standard protocols / rapid ratio imaging / online ratio imaging / experimental applications / real time / media containing ionomycin / acquisition software / software package / imaging / aqueous solution / fluorescence resonance energy transfer measurements using fluorescence microscopy / fluorescence resonance-energy transfer / online ratio images / chemical reactions / equivalent microscope systems / online ratioing / fluorescence resonance energy transfer / similar protocol / equivalent software / Optical imaging / software program / offline data processing / data-processing program / be reanalyzed using acquisition software / /

NaturalFeature

YFP channel / FRET channel / /

Organization

National Institute of Health / University of Colorado / Pharmacology and Howard Hughes Medical Institute / University of Colorado / Boulder / University of California / San Diego / /

Person

Venus References / Robert / V / Nat / Ruth L. Kirschstein / /

Position

investigator / Prime Minister / researcher / Representative / controller / /

Product

Ca2+ / Lambda / balanced salt / OPTI / Ca2 / G418 / /

ProvinceOrState

Oregon / New York / North Carolina / California / Colorado / /

PublishedMedium

NATURE PROTOCOLS / /

Technology

1061 PROTOCOL / 550 PROTOCOL / Cloning / specific protocol / 1057 PROTOCOL / transgenic organisms / ASCII / NATURE PROTOCOLS / 1063 PROTOCOL / imaging protocol / similar protocol / /

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http /

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