RNAi by feeding 2 Steps 1. Transform L4440-XX plasmid into HT115 (DE3) bacteria – plate on LB amp tet 2. Inoculate 50 ml LB amp tet w. 0.5 ml O N culture or directly from plate 3. Grow at 37°C until OD600 reaches 0.4 - Bruce Li - Document - PDFSEARCH.IO

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Date: 2012-09-17 09:29:00		RNAi by feeding 2 Steps 1. Transform L4440-XX plasmid into HT115 (DE3) bacteria – plate on LB/amp/tet 2. Inoculate 50 ml LB/amp/tet w. 0.5 ml O/N culture or directly from plate 3. Grow at 37°C until OD600 reaches 0.4 Add to Reading List Source URL: hymanlab.mpi-cbg.de Download Document from Source Website File Size: 113,89 KB Share Document on Facebook