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WHOLE-MOUNT DOUBLE IN SITU HYBRIDIZATION OF ZEBRAFISH EMBRYOS Fix embryos in prefered fixative (4% PFA is the standard) for 3h at RT or overnight at 4˚C. Wash with 50% MeOH/PBST Wash 2x with 100% MeOH Store at -20˚C at
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Document Date: 2013-04-16 12:52:24
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WHOLE-MOUNT DOUBLE IN SITU HYBRIDIZATION OF ZEBRAFISH EMBRYOS Fix embryos in prefered fixative (4% PFA is the standard) for 3h at RT or overnight at 4˚C. Wash with 50% MeOH/PBST Wash 2x with 100% MeOH Store at -20˚C at