Fractionation of Membrance Cytoplasmic and Nuclear Proteins

First Page		Document Content
Date: 2014-07-24 01:52:24 Cell biology Protein methods Centrifugation Proteins Protein purification PMSF Lysis buffer Triton X-100 Pepstatin Chemistry Biology Laboratory techniques		Fractionation of Membrance Cytoplasmic and Nuclear Proteins Add to Reading List Source URL: www.genetex.com Download Document from Source Website File Size: 1,44 MB Share Document on Facebook

	RESEARCH ARTICLES ␮g/ml pepstatin, 4 mM PMSF, and 0.5% NP-40) was then added. The tubes were shaken for 2 hours in the The supernatant was collected for ELISA and protein measurements. The ELISA reaction was completed DocID: 1nxQ6 - View Document
	Dr. Daniel H. Rich School of Pharmacy University of Wisconsin-Madison 777 Highland Avenue Madison, WITelephone: DocID: 13Uvu - View Document
	Acetate-induced apoptosis in colorectal carcinoma cells involves lysosomal membrane permeabilization and cathepsin D release DocID: ZADM - View Document
	Fractionation of Membrance Cytoplasmic and Nuclear Proteins DocID: YIIS - View Document
	Protein Extraction of Yeast Cells, Denaturing Vortex Method -Collect 10mL culture over 2-3mL ice in culture tube. OD660 ∼0.2 in Genesys Spec20 (∼1 - 2*107 cells/ml) -Pellet 2000RPM, 2 min, 4°C and pour off supern DocID: whL1 - View Document