gRNA Synthesis Protocol STEP 1: Find all 23bp genomic sites of the form 5’-N20NGG-3’ near your intended target site (ideally ±50bp). These may reside on the or – strand. PAM 5’!NNNNN NNNNN NNNNN NNNNN NGG!3’ - DNA synthesis - Document - PDFSEARCH.IO

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Date: 2014-11-05 23:32:09 Chemistry Guide RNA Polymerase chain reaction Oligonucleotide Molecular cloning Vector DNA microarray Gene expression Biology Molecular biology Biochemistry		gRNA Synthesis Protocol STEP 1: Find all 23bp genomic sites of the form 5’-N20NGG-3’ near your intended target site (ideally ±50bp). These may reside on the + or – strand. PAM 5’!NNNNN NNNNN NNNNN NNNNN NGG!3’ Add to Reading List Source URL: www.addgene.org Download Document from Source Website File Size: 115,20 KB Share Document on Facebook

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