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Biochemistry / Laboratory techniques / Polymerase chain reaction / DNA / RNA / Real-time polymerase chain reaction / Gene expression / Reverse transcription polymerase chain reaction / Fluorescence in situ hybridization / Biology / Molecular biology / Chemistry


Anal Bioanal Chem[removed]:3383–3393 DOI[removed]s00216[removed]ORIGINAL PAPER Quantifying mRNA levels across tissue sections
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Document Date: 2011-07-13 14:57:51


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Company

Biosearch Technologies / Qiagen / /

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Event

FDA Phase / /

Facility

College Park / National Cancer Institute / University of Maryland / Laboratory of Pathology / /

IndustryTerm

purification protocol / rubber pad / online version / multi-well array device / chemical lysis / Web application / similar parallel-processing methodology / parallel processing / buckm@mail.nih.gov applications / nucleic acid purification protocol / sufficient amplified product / magnetic bead solution / scaled-down protocol / lysis solution / higher throughput sample processing / microfluidic sample processing chips / generated using the Primer3 Web application / /

MedicalCondition

J Cancer / breast carcinomas / breast cancer / disease / node-negative breast cancer / papilloma virus / /

Organization

National Cancer Institute / National Institute of Health / Laboratory of Pathology / B. Shapiro Fischell Department of Bio-Engineering / University of Maryland / College Park / Center for Cancer Research / Department of Mechanical Engineering / Pathogenetics Unit / /

Person

Michael R. Emmert-Buck / Michael Armani / Arch Pathol / Elisabeth Smela / Michael A. Tangrea / Connie Rogers-Newcome / Benjamin Shapiro / /

Position

Walker / Wencyk PM / pathologist / Fisher / /

Product

acetic acid / Cts / nucleic / HPRT1 / Nucleic acid / AM1005M / /

ProvinceOrState

Maryland / /

PublishedMedium

Cancer Research / /

Technology

scaled-down volume ChargeSwitch protocol / nucleic acid purification protocol / onestep RT-qPCR protocol / scaled-down protocol / hybridization / unmodified ChargeSwitch protocol / PicoPure protocol / DNA binding / described protocol / one-tube protocol / ChargeSwitch protocol / detailed protocol / Laser / 2D-RT-qPCR protocol / purification protocol / Gene expression / condensation / unmodified CS protocol / microfluidic sample processing chips / following protocol / 20-fold scaled-down protocol / same protocol / parallel processing / /

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