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Gene delivery / Microbiology / Molecular genetics / CRISPR / Guide RNA / Delitto perfetto / Plasmid / Knockout rat / Vector / Biology / Molecular biology / Genetics


Nucleic Acids Research Advance Access published March 4, 2013 Nucleic Acids Research, 2013, 1–8 doi:[removed]nar/gkt135 Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
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Norville / Coralville / Boston / YPAD / L.A. / Durham / /

Company

R. (2011) CRISPR-Cas Systems / T.G. (2000) DNASTAR / Phusion 2X HF / Oxford University Press / Lasergene Seqman Pro Software / Cox / Integrated DNA Technologies / Creative Commons / /

Country

Mali / United States / /

Currency

pence / /

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Facility

Boston University / Cas9 complex / RNA scaffold / /

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IndustryTerm

immune systems / large gene networks / media containing canavanine / thialysine containing media / sequence analysis software / selective media / media plates / dropout media / rst round product / biotechnology community / non-selective media / foundational tool / genomic target site / oligonucleotide-targeting site / target site / genome engineering tool / defined media / uracil media / endonuclease site / cut site / putative cleavage site / /

Organization

Harvard Medical School / NEB Gibson Assembly / Department of Biomedical Engineering / National Science Foundation / Department of Energy / Boston University / Boston / Synthetic Biology Engineering Research Center / Oxford University / /

Person

Harris Wang / Nikolai Eroshenko / Microbiol / Nat / Fred Winston / Gonzales / Dan Spatt / Alejandro Chavez / /

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Position

General / Author / designer / positively selectable reporter / reporter / /

Product

SC / Sigma / Dextrose / Burland / Sorbitol / /

ProvinceOrState

South Carolina / Massachusetts / /

PublishedMedium

Nucleic Acids Research / /

Technology

biotechnology / DNA binding / human genome / ATG / Electroporation / site-specific mutagenesis / gene targeting / recombination / /

URL

http /

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