PROTOCOL A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis

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Date: 2009-02-17 05:25:46 Molecular biology Biotechnology Genetics Stem cells Genetically modified organisms Invitrogen Puromycin Gene knockout Stem-cell line Gene targeting Molecular cloning Polymerase chain reaction		PROTOCOL A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis Add to Reading List Source URL: www.fbae.org Download Document from Source Website File Size: 441,70 KB Share Document on Facebook

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	Total time 6.5 hours Procedures Lipofectamine 2000 method (Invitrogen) (for 1 well in 6-well cluster 10cm2, small dish of 35 mm). DocID: 1v0KO - View Document
	PROTOCOL A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis DocID: 1oXs8 - View Document
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