Transformation Procedure 1. Take ice, put DH5a, wait to thaw 2. Aliquote 50 ul to prechilled 600 ul tubes 3. Add 1 ul DNA to tubes and wait for 30 min on ice. 4. Heat shock 42oC for 45 sec. 5. Incubate on ice for 2 min

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Date: 2017-09-15 13:58:59		Transformation Procedure 1. Take ice, put DH5a, wait to thaw 2. Aliquote 50 ul to prechilled 600 ul tubes 3. Add 1 ul DNA to tubes and wait for 30 min on ice. 4. Heat shock 42oC for 45 sec. 5. Incubate on ice for 2 min Add to Reading List Source URL: wang.eng.ucsd.edu Download Document from Source Website File Size: 42,18 KB Share Document on Facebook