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Molecular biology / Laboratory techniques / Gene expression / Real-time polymerase chain reaction / Polymerase chain reaction / Cell culture / Cell biology / Life Technologies / Fluorescence in situ hybridization / Biology / Chemistry / Science


A reinvestigation of somatic hypermethylation at the PTEN CpG island in cancer cell lines
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Arizona State University / The Biodesign Institute / Cell Imaging Station / /

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step-wise protocol / cell imaging / microscopic imaging / diluted cell solution / biological systems / difficult manipulation tools / nitrogen gas / Online METHODOLOGY Open Access / downstream applications / chemical dissociation / chemical manipulation / online submission / experimental protocols / /

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chemical dissociation / neurodegenerative and neuropsychiatric disorders / Confirm sterility / dissociation / cancer / disease / tumour / minimal residual cancer / /

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National Institute of Health / Microscale Life Sciences Center / Arizona State University / Biodesign Institute / Center for Biosignatures Discovery Automation / NIH Center of Excellence / /

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Paul Terasaki / Jia Zeng / Valerie Harris / /

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gene amplification / detailed / step-wise protocol / cloning / stem cells / Microfluidics / Laser / gene expression / DNA sequencing / presented protocol / step-wise protocol / gel electrophoresis / /

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