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Protein Extraction of Yeast Cells, Denaturing Vortex Method -Collect 10mL culture over 2-3mL ice in culture tube. OD660 ∼0.2 in Genesys Spec20 (∼1 - 2*107 cells/ml) -Pellet 2000RPM, 2 min, 4°C and pour off supern
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Document Date: 2008-12-10 14:02:21
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File Size: 37,00 KB
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Equilibrium chemistry
Peptides
Amines
SDS-PAGE
Buffer solution
Tris
PH
Leupeptin
Pepstatin
Chemistry