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Biochemistry / RNA / Molecular biology / DNA / Lac operon / RNA interference / RNA polymerase / Non-coding RNA / CRISPR / Biology / Gene expression / Genetics


Resource Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression Lei S. Qi,1,2,8,* Matthew H. Larson,2,3,8 Luke A. Gilbert,2,3,8 Jennifer A. Doudna,4,5,6,8,9 Jonathan S. Weissman,2,
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Document Date: 2014-12-24 04:42:16


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San Francisco / U6 / /

Company

Engineering Transcriptional Regulatory Networks / Sigma Aldrich / Elsevier Inc. / QIAGEN / De Novo Software / GE Healthcare / BD Biosciences / Lawrence Berkeley National Laboratory / /

Country

Mali / United States / /

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pence / /

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Product Issues / /

Facility

CRP complex / RNA complex / Bioengineering University of California / Scale bar / Hughes Medical Institute University of California / terminal SV40 / DNA Sequencing The DNA library / /

IndustryTerm

clinical applications / cellular systems / proper host machinery / ice-cold precipitation solution / lactose regulatory network / machinery / transcription start site / established library generation protocol / microbial gene networks / endogenous gene networks / gene knockdown tool / regulatory machinery / transcription operator site / transcription pause site / target site / transcriptional regulatory networks / oligonucleotide synthesis technology / regulatory network / annotated transcription start site / alkaline fragmentation solution / cis-regulatory site / chemical / cis regulatory site / pause site / /

MedicalCondition

retroviral infection / viral infection / /

Organization

Wendell A. Lim1 / 2 / 3 / 8 1UCSF Center for Systems / University of California / Berkeley / USA 8California Institute for Quantitative Biomedical Research / University of California / San Francisco / San Francisco / /

Person

Gene Knockdown Is Inducible / Matthew H. Larson / Reversible Unlike / Gene Knockdown / Lei S. Qi / Gene Silencing / /

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Position

eNT2 eNT3 eNT4 eT1 eT2 eNT5 EGFP EGFP reporter / bp chromosomal reporter / control reporter / already transcribed messenger / reporter / /

Product

kanamycin / penicillin / ampicillin / CRISPRi / Cas9 / sgRNAs / chloramphenicol / dcas9 protein / /

ProgrammingLanguage

Python / /

ProvinceOrState

California / /

PublishedMedium

Molecular and Cellular Biology / /

Technology

genetic engineering / Cloning / DNA binding / library generation protocol / ATG / electroporation / Gene Expression / DNA Sequencing / gene targeting / recombination / large-scale DNA oligonucleotide synthesis technology / /

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http /

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