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Chemistry / RNA / Gene expression / Laboratory techniques / Transcriptome / RNA-Seq / Reverse transcription polymerase chain reaction / Cell biology / Chromatin immunoprecipitation / Biology / Biochemistry / Molecular biology


MARIS: Method for Analyzing RNA following Intracellular Sorting Sinisˇa Hrvatin1, Francis Deng1, Charles W. O’Donnell1,2, David K. Gifford2, Douglas A. Melton1,3* 1 The Harvard Department of Stem Cell and Regenerative
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Document Date: 2014-05-21 15:11:47

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extraction protocol / Nature biotechnology / pancreatic differentiation protocol / chemical fixation / optimized existing protocols / biophysical research communications / differentiation protocol / reagents/materials/analysis tools / directed differentiation protocols / /

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Harvard University / Melton DA / Harvard Stem Cell Institute / University of Tampere / Harvard Department of Stem Cell and Regenerative Biology / Massachusetts Institute of Technology / Harvard University Committee on the Use of Human Subjects / Howard Hughes Medical Institute / /

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Genomics / Stepwise directed differentiation protocols / Antibodies / Hybridization / biotechnology / manufacturer-recommended protocol / transplantation / stem cells / RNA-Seq technology / Illumina TruSeq protocol / human genome / optimized existing protocols / developed protocol / laser / cell RNASeq protocols / DNA Chip / RNA extraction protocol / Supporting Information Figure S1 Directed differentiation protocol / gene expression / pancreatic differentiation protocol / /

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