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Plasmid / Protein expression / Transformation / Subcloning / Polymerase chain reaction / Molecular cloning / Recombinant DNA / Shuttle vector / Natural competence / Biology / Molecular biology / Bacillus subtilis


Simple, fast and highefficiency transformation system for directed evolution of cellulase in Bacillus subtilis
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Document Date: 2010-11-23 08:40:58


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City

Ehrlich / Carlsbad / Blacksburg / Giles / Chichester / Bryan / References Amin / New York / Madison / Pittsburgh / Louis / Ridge / St. Louis / /

Company

GenBank / Blackwell Publishing Ltd / DuPont / Integrated DNA Technologies / Cold Spring Harbor Laboratory / John Wiley & Sons / /

Country

United States / United Kingdom / /

Currency

pence / /

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Event

FDA Phase / /

Facility

College of Agriculture / Bacillus Genetic Stock Center / Bscel5 library / Bioprocessing Research Center / Virginia Polytechnic Institute / State University / /

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IndustryTerm

minimal media / easy and robust transformation protocol / machinery / routine protocol / restriction enzyme cutting site / integration site / modified protocol / multimerization products / chemicals / /

MedicalTreatment

antibiotics / /

MusicGroup

Bscel5 / /

Organization

Department of Biological Systems Engineering / Bacillus Genetic Stock Center / Society for Applied Microbiology and Blackwell Publishing Ltd / Department of Energy / USDA Bioprocessing and Biodesign Center / office of Biological and Environmental Research / BioEnergy Science Center / U.S. Securities and Exchange Commission / College of Agriculture / DOE BioEnergy Science Center / Polytechnic Institute / College of Agriculture and Life Sciences Biodesign / State University / Life Sciences Biodesign and Bioprocessing Research Center / Virginia Tech / Institute for Critical Technology and Applied Science / US Department of Energy Bioenergy Research Center / DOE office of Science / Society for Applied Microbiology / /

Person

Gallagher / Daniel Zeigler / Fisher Scienti / Sha / /

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Position

Professor / Fisher / /

Product

kanamycin / ampicillin / erythromycin / xylose / M-9 / chloramphenicol / Shafikhani / /

ProvinceOrState

Virginia / South Dakota / Tennessee / P.N. / Nova Scotia / New Hampshire / /

PublishedMedium

The Authors Journal / Environmental Research / /

Technology

Proteomics / electrophoresis / modified protocol / Biotechnology / polymerization / DNA shuffling / cloning / Shafikhani protocol / transformation protocol / routine protocol / electroporation / directed evolution / DNA sequencing / Bioprocessing / recombination / gel electrophoresis / /

URL

http /

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