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City San Francisco / xCELLigence Impedance Array / Houston / / Company Stem Cell Technologies / ACEA Biosciences / Jackson Laboratory / Nikon / Bangs Laboratories / Takara / PLGA / Corning / CellASIC/EMD Biosciences / / Facility Stable Cell Line Generation / University of North Carolina Medical School / M. D. Anderson Cancer Center / Jackson Laboratory / Chapel Hill / University of California / / IndustryTerm polymer solution / cell-tracking software / oil-in-water emulsion method / transfection solution / preclinical imaging system / sterile-filtered chemoattractant solution / Time-lapse imaging / chemoattractant solutions / microfluidic chemotaxis device / receptor processing / Chemical structure / imaging / mg/mL solution / buffered saline solution / microfluidic chemotaxis assay device / In vivo bioluminescent imaging / / MedicalCondition cancer / JE / infection / MS / / Organization African Union / FDA / University of North Carolina Medical School / University of California / San Francisco / UCSF Institutional Animal Care and Use Committee / Drug Studies Unit / / Person Bryan Roth / Brian Rabinovich / / Position designer / representative / / Product potassium chloride / clozapine / acetylcysteine / sodium chloride / Sigma / / ProvinceOrState California / / RadioStation Calcein AM / / Technology Electroporation / leukoreduction / antibodies / Biotechnology / http / cloning / Cell Signaling / same protocol / recombination / flash / quantitated using fluorescent secondary antibodies / / URL www.pnas.org/cgi/content/short/1402087111 / / SocialTag 
Supporting Information Park et alpnasSI Materials and Methods Gene Constructs. The DREADD (designer receptor exclusively activated by a designer drug) constructs hM3Dq, hM3(M2i3)Di, and hM4Di were a