JOURU OF BACTERIOLOGY, Dec. 1992, p[removed]Vol. 174, No. 24

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Microbiology Mobile genetic elements Gene delivery Enterobacteria PUC19 Restriction enzyme Plasmid Transformation Escherichia coli Biology Bacteria Molecular biology		JOURU OF BACTERIOLOGY, Dec. 1992, p[removed]Vol. 174, No. 24 Add to Reading List Source URL: www.ncbi.nlm.nih.gov Download Document from Source Website File Size: 1,40 MB Share Document on Facebook

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	RNAi by feeding 2 Steps 1. Transform L4440-XX plasmid into HT115 (DE3) bacteria – plate on LB/amp/tet 2. Inoculate 50 ml LB/amp/tet w. 0.5 ml O/N culture or directly from plate 3. Grow at 37°C until OD600 reaches 0.4 DocID: 1vadj - View Document