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doi:nature08187 LETTERS Programming cells by multiplex genome engineering and accelerated evolution Harris H. Wang1,2,3*, Farren J. Isaacs1*, Peter A. Carr4,5, Zachary Z. Sun6, George Xu6, Craig R. Forest7
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Document Date: 2009-08-28 17:00:38


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Boston / Cambridge / Sharan / /

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Macmillan Publishers Limited. / F. H. Laboratory / Thomason L. C. / Bio-Rad / Integrated DNA Technologies / /

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United States / /

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FDA Phase / /

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Plant Cell / Church laboratory / Wyss Institute / Georgia Institute of Technology / Massachusetts Institute of Technology / Harvard University / /

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chemical synthesis / Exchange media / Wash away media / automation device / supernatant lycopene solution / free energy / online version / gene target search / supernatant media / animal systems / extracted lycopene solution / degenerate ribosome binding site / hybridization free energy / prototype device / hybridization energy / allelic replacement protocol / industrial and pharmaceutical relevance24 / web server / prototype devices / gene networks / /

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A. D. / /

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Sargasso Sea / /

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4The Center for Bits and Atoms / Harvard Medical School / National Institute of Health / Harvard University / National Science Foundation / Wyss Institute for Biologically Inspired Engineering / D. Court / NDSEG / 5Media Lab / Massachusetts Institute of Technology / Department of Genetics / 7George W. Woodruff School of Mechanical Engineering / Georgia Institute of Technology / Atlanta / MIT Division of Health Sciences and Technology / 6Harvard College / /

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Pitera / Smolke / Court / J. Jacobson / /

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D. J. / Farmer / Author Information Reprints / Author / /

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kanamycin / ampicillin / chloramphenicol / MG1655 / EcNR2 / /

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S. K. / Georgia / Massachusetts / /

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Nature Protocols / /

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genetic engineering / cloning-based technologies / hybridization / DNA shuffling / cloning / HTML / Aeration / MAGE technology / drug discovery / electroporation / directed evolution / genetic code / allelic replacement protocol / DNA sequencing / recombination system / electroporation system / genotype / recombination / web server / using recombination in Escherichia coli / /

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www.nature.com/nature / www.nature.com/reprints / www.nature.com / /

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