Yeast Two-Hybrid Procedure 1. Generate a GAL4 DNA-BD fusion by cloning the gene of interest in frame with the GAL4 DNA binding domain of pGBKT7. 2. Transform AH109 with bait plasmid, detect bait expression and test for a - Plasmid - Document - PDFSEARCH.IO

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	Yeast Two-Hybrid Procedure 1. Generate a GAL4 DNA-BD fusion by cloning the gene of interest in frame with the GAL4 DNA binding domain of pGBKT7. 2. Transform AH109 with bait plasmid, detect bait expression and test for a Add to Reading List Open Document File Size: 85,52 KB Share Result on Facebook

Yeast Two-Hybrid Procedure 1. Generate a GAL4 DNA-BD fusion by cloning the gene of interest in frame with the GAL4 DNA binding domain of pGBKT7. 2. Transform AH109 with bait plasmid, detect bait expression and test for aAdd to Reading List