IgG / Heinz / TyrH52 / TyrL32 / Silicon Graphics / Russell / ErikssonA.E. / Oxford University Press / Wilson / AspH56 / A 1 B 5 AB / Pharmacia / GruenL.C. / Fv / /
Country
Sweden / United States / United Kingdom / /
Currency
pence / /
Facility
Research Institute / Rex Richards Building / NA complex / Laboratory of Molecular Biophysics / LaTrobe University / /
IndustryTerm
free energy change / side chain hydroxyl groups / free energy / charged groups / charged side chain / antibody heavy chain / larger antibody side chain / energy loss / antibody light chain / hydrophobic / uncharged side chain / restriction endonuclease site / heavy and light chain / software package / side chain / energy penalties / energy / larger protein side chain / methylene / threonine side chain / mutation site / free energy model / gas constant / antibody binding site / overall free energy / restriction site / hydrophilic / sensor chip / /
OperatingSystem
L3 / /
Organization
1Biomolecular Research Institute / National Institute of Health / US Public Health Service / Laboratory of Molecular Biophysics / Human Service / Diagnostic Technologies and 1Biomolecular Research Institute / Oxford University / Department of Health / Environmental Protection Agency / LaTrobe University / /
Person
David A.Dougan / Harley / V / Colman / Alexander A.Kortt / Fab / J.E.Burns / R.G.Webster / Lawrence / D.W.Metzger / Hudson / D.Hewish / L.Pearce / L.Clem Gruen / / /
Position
Reverse primer AspH56Ala AspH56Ala AspH56Ser AspH56Ser AspH56Gly AspH56Gly AspH56Asn AspH56Asn AspH56Glu AspH56Glu TyrH99Ala TyrH99Ala TyrH100aPhe TyrL32Phe TyrL32Phe ThrL93Trp Forward primer Reverse primer ThrL93Trp ThrL93Phe Forward / representative set / primer Reverse primer ThrL93Trp ThrL93Phe Forward primer Reverse primer LeuL94Trp Forward / primer Reverse primer Forward primer Reverse primer ThrL93Phe LeuL94Tyr LeuL94Val LeuL94Val LeuL94Ala LeuL94Ala Forward / primer Forward primer Reverse primer Forward primer Forward / primer Reverse primer Forward primer Reverse primer Forward / King / VL Forward / primer Forward primer Reverse primer AspH56Ala AspH56Ala AspH56Ser AspH56Ser AspH56Gly AspH56Gly AspH56Asn AspH56Asn AspH56Glu AspH56Glu TyrH99Ala TyrH99Ala TyrH100aPhe TyrL32Phe TyrL32Phe ThrL93Trp Forward / representative / primer Reverse primer LeuL94Trp Forward primer Reverse primer LeuL94Trp LeuL94Tyr Forward / primer LeuL94Trp LeuL94Tyr Forward primer Forward primer Reverse primer Forward / Sheriff / primer LeuL94Trp Forward primer Reverse primer LeuL94Trp LeuL94Tyr Forward primer Forward / Reverse primer Forward primer Reverse primer Forward / /
Protein Engineering vol.11 no.1 pp.65–74, 1998 Effects of substitutions in the binding surface of an antibody on antigen affinity David A.Dougan, Robyn L.Malby1,2, L.Clem Gruen,