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Medicine / Laboratory techniques / Molecular biology / Animal diseases / Scrapie / Prions / Transmissible mink encephalopathy / Protein Misfolding Cyclic Amplification / Creutzfeldt–Jakob disease / Transmissible spongiform encephalopathies / Biology / Health


Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays Jason M. Wilham1, Christina D. Orru´1,2, Richard A. Bessen3, Ryuichiro Atarashi4, Kazunori Sano4, Brent Race1, Kimberly D.
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Document Date: 2014-09-19 14:47:08


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File Size: 2,51 MB

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City

Bozeman / Hamilton / Gilroy / Monserrato / /

Company

EMD Biosciences / Rocky Mountain Laboratories / RT-QuIC / Simonsen Laboratories / Qiagen / Creative Commons / GE Healthcare / On-Line Instrument Systems Inc. / /

Country

Italy / United States / /

Currency

USD / /

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Facility

National Institute of Allergy / University of Cagliari / Laboratory Animal Care / Laboratory of Persistent Viral Diseases / Montana State University / Laboratory Animals / University of Alberta / /

IndustryTerm

amyloid product / carrier protein / reaction products / important applications / constant nitrogen gas purge / protease-resistant prion-seeded products / polypeptide products / mg/mL solution / /

MedicalCondition

263K scrapie / few mouse-adapted scrapie / strain / sporadic CJD / human Creutzfeldt-Jakob disease / transmissible mink encephalopathy / prion disease infections / certain murine-adapted scrapie / HY TME infection / prion disease / original scrapie / 263K hamster scrapie / fatal neurodegenerative disorders / normal and clinical 263K scrapie / infectious agent / infections / infectious prions / standard scrapie cell assay / hamster 263K scrapie BH / Summary Prion diseases / scrapie BH / disease / chronic wasting disease / i.c. scrapie / hamster scrapie BH / prion diseases / scrapie / Introduction The transmissible spongiform encephalopathies / diseases / variant CJD / sheep scrapie / 263K scrapie BH / bovine spongiform encephalopathy / infectious neurodegenerative disorders / infectious particles / /

MedicalTreatment

surgery / dialysis / /

Organization

National Institute of Health / University of Cagliari / Department of Molecular Microbiology and Immunology / University of Alberta / Department of Biomedical Sciences and Technologies / Nagasaki University Graduate School / Animal Use and Care Committee / Montana State University / Association for Assessment of Laboratory Animal Care / National Institute of Allergy and Infectious Disease / Laboratory of Persistent Viral Diseases / /

Person

Elmer Spectrum / Christina D. Orru / David Westaway / /

Position

Editor / RT / Author / representative / /

Product

RTQuIC / SD50 / guanidine hydrochloride / isoflurane / Spearman-Ka / Overnight Express Autoinduction system / Overnight Express Autoinduction / /

ProvinceOrState

Alberta / Montana / /

PublishedMedium

PLoS Pathogens / the PLoS Pathogens / /

Technology

alpha / polymerization / prions / Spectroscopy / genotype / dialysis / /

URL

www.plospathogens.org / /

SocialTag