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IP followed by western blot Every step needs to be performed cold, either in the cold room or on ice!!! • Lyse cells in appropriate lysis buffer (standard buffer is 1% NP40 or Triton-X100) with protease inhibitors
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Document Date: 2016-06-16 10:34:43
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UPDATE
IP followed by western blot Every step needs to be performed cold, either in the cold room or on ice!!! • Lyse cells in appropriate lysis buffer (standard buffer is 1% NP40 or Triton-X100) with protease inhibitors