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Protein methods / Immunologic tests / Protein-protein interaction assays / Microarrays / Chromatin immunoprecipitation / Molecular biology / ChIP-on-chip / Fixation / Chip-sequencing / Biology / Laboratory techniques / Biochemistry


truChIP Application Note www.covarisinc.com Optimizing sample fixation and chromatin shearing for
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Document Date: 2014-08-19 05:45:46

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City

Billerica / Santa Clara / Bethesda / Woburn / New York / /

Company

Qiagen / IgG / AFA / Millipore / Covaris Inc. / Agilent / /

Facility

NIH Campus / Laboratory of Immunology / Plant Methods / /

IndustryTerm

energy passing / formaldehyde solutions / sample over-processing / excess energy / acoustic energy / higher acoustic energy / mechanical shearing technology / truChIP protocol / formaldehyde solution / high-frequency acoustic energy / optimized energy / downstream processing / quenched fixation solution / acoustic technology / determined binding site / controlled processing / ultrasonic energy / microarray chip / energy / /

Organization

National Institute of Health / Mount Sinai School of Medicine / FDA / Division of Therapeutic Proteins / Laboratory of Immunology / /

Person

Dan Hasson / Anna Krowczynska / Alicia Alonso / Gary Smejkal / Frederick C. Mills / Hamid Khoja / Ralph M Bernstein / /

ProvinceOrState

Maryland / New York / Massachusetts / /

PublishedMedium

Nature Protocols / /

Technology

DNA Chip / antibodies / polymerization / mechanical shearing technology / Nature Protocols / ultrasound / recombination / gel electrophoresis / truChIP protocol / detailed protocol / then carried out using specific antibodies / /

URL

www.covarisinc.com / http /

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