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City New York / Alphavirus Particle / Basel / / Company IgG / NIH Risk Group / Improved Immune Induction Using Electroporation Loading EP LLC / MaxCyte Inc. / Humana Press / / Continent Asia / North America / / Country Switzerland / Japan / United States / / Currency pence / / / Event Product Issues / / IndustryTerm cellular immunotherapy product / egg-based manufacturing / transfection protocols / chemical-based transient transfection / media additives / flow electroporation technology / Culture media / expression systems / downstream processing / cell type-specific electroporation protocols / control / Treatment of Advanced Leukemia / transfection technologies / chemical reagents / large-scale lentivector manufacturing / vaccine applications / Treatment of Cancer / cellular expression systems / gene therapy products / cell therapeutic applications / benchtop flow electroporation systems / clinical and commercial applications / manufacturing / chemical transfection methods / chemical methods / biodefense applications / / MedicalCondition host cell infection / stringent sterility / HIV / whole cell tumor / anti-solid tumor / smallpox / baculovirus infection / swine H1N1 flu / progressive tumor / solid tumors / ORF / hepatitis B / 51Cr-labeled intact LLC tumor / subsequent infection / Lewis lung carcinoma / B. Flank tumors / influenza / considerably less tumor / NOD/scid / cancers / tumor / infectious material / significant tumor / cancer / Human Disseminated Tumor / Tumors / therapeutic metastatic tumor / extracellular tumor / Advanced Leukemia / infectious titers / Ebola / alphavirus infection / established mesothelin-positive tumors / solid tumor / specific tumor / inflammatory disorders / Avian H5N1 Influenza / polio / B16 melanoma / CAR Expresion B. Tumor / human papilloma virus / diseases / whole tumor / infectious diseases / infection / / MedicalTreatment immunization / Adoptive transfer / immunotherapies / vaccination / radioimmunotherapy / adoptive immunotherapy / immunotherapy / gene therapy / cell therapy / / Organization National Institute of Health / US Federal Reserve / FDA / / Person CHO CHO HEK / James Brady / Gene Ther / Madhusudan V. Peshwa / Interest Cells Animal-derived / Krista Steger / Weili Wong / Interest Cells / Meg Duskin / Li Linhong / Karen Donato / / / Position High Performance Transient Transfection General / / Product M108 injection / MaxCyte STX / Pyle P32 Television / vectors / / ProvinceOrState Maryland / / PublishedMedium PLoS ONE / Molecular Biology / / Technology biomanufacturing / cell therapy / transfection protocols / siRNA / antibodies / cell type-specific electroporation protocols / Stem Cells / gene delivery vectors / Transient transfection technologies / Recombinant Protein Production / gene therapy / gene cloning / STX protocol / Electroporation / gene expression / flow electroporation technology / at simplifying gene cloning / / URL http / SocialTag 
Flow Electroporation for Vaccine Development and Production: From Subunit Vaccines to Ex Vivo Immunotherapy Contributing Authors: Krista Steger, Ph.D., James Brady, Ph.D., Weili Wong, Ph.D., Li Linhong, Ph.D., Meg Duskin