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Molecular biology / Polymerase chain reaction / DNA replication / Laboratory techniques / DNA / Primer / DNA polymerase / Taq polymerase / Real-time polymerase chain reaction / Biology / Chemistry / Biochemistry


Article pubs.acs.org/biochemistry Directed Evolution of Polymerases To Accept Nucleotides with Nonstandard Hydrogen Bond Patterns Roberto Laos,† Ryan Shaw,† Nicole A. Leal,† Eric Gaucher,‡ and Steven Benner*,†
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Document Date: 2015-04-24 17:42:14


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File Size: 3,90 MB

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City

Gainesville / Coralville / Rappaport / Washington / DC / /

Company

Jet Propulsion Laboratory / Mitsui / Matsuda / Ho D. D. / Benner S. A. / AEGIS / Havemann S. A. / Shen L. P. / Integrated DNA Technologies / Leal N. A. / ASSOCIATED CONTENT / /

Country

United States / /

Currency

pence / /

Event

Movie Release / /

Facility

Library Creation / Georgia Institute of Technology / duplex DNA / Parker H. Petit Institute / NASA Astrobiology Institute / The Westheimer Institute of Science / /

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IndustryTerm

water-in-oil-emulsion / light mineral oil / oil / aqueous solution / extended product / water-in-oil emulsions / genetic expanded information systems / triphosphate binding site / genetic systems / individual site / dZ site / /

Movie

A. D. / A. I. / /

Organization

Chemistry / and Parker H. Petit Institute for Bioengineering and Biosciences / School of Biology / Defense Threat Reduction Agency / School of Chemistry / NASA Astrobiology Institute / Westheimer Institute of Science and Technology / Foundation for Applied Molecular Evolution / American Chemical Society / Vol. / National Aeronautics and Space Administration / Holliger laboratory / Georgia Institute of Technology / Atlanta / /

Person

Eric Gaucher / Nat / Zunyi Yang / Nicole A. Leal / † Eric / Steven Benner / Ryan Shaw / † Nicole / Clement Vulin / Shuichi Hoshika / /

/

Position

thermal cycler / AUTHOR INFORMATION Corresponding Author *E-mail / designer / /

Product

Biosystems ABI394 DNA/RNA / Expanded Genetic Information System / /

ProvinceOrState

Florida / Georgia / /

PublishedMedium

The AEGIS / /

Technology

Protein Engineering / polymerization / directed evolution / genetic code / SNP / Molecular Evolution / genotype / gel electrophoresis / /

URL

http /

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