Thaw

Results: 267



#Item
1Transformation Procedure 1. Take ice, put DH5a, wait to thaw 2. Aliquote 50 ul to prechilled 600 ul tubes 3. Add 1 ul DNA to tubes and wait for 30 min on ice. 4. Heat shock 42oC for 45 sec. 5. Incubate on ice for 2 min

Transformation Procedure 1. Take ice, put DH5a, wait to thaw 2. Aliquote 50 ul to prechilled 600 ul tubes 3. Add 1 ul DNA to tubes and wait for 30 min on ice. 4. Heat shock 42oC for 45 sec. 5. Incubate on ice for 2 min

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Source URL: wang.eng.ucsd.edu

Language: English - Date: 2017-09-15 13:58:59
    2ORIGINAL RESEARCH ARTICLE published: 11 December 2014 doi: fmicbSpring thaw ionic pulses boost nutrient availability and microbial growth in entombed Antarctic Dry Valley

    ORIGINAL RESEARCH ARTICLE published: 11 December 2014 doi: fmicbSpring thaw ionic pulses boost nutrient availability and microbial growth in entombed Antarctic Dry Valley

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    Source URL: glaciers.pdx.edu

    Language: English - Date: 2017-09-23 19:11:43
      3Bacteria transformation spin down → 1 μl add 9 μl H2O thaw chemo-competent E.coli SURE cells (Stratagene) on ice take 40 μl cells and add 1 μl of the dilution

      Bacteria transformation spin down → 1 μl add 9 μl H2O thaw chemo-competent E.coli SURE cells (Stratagene) on ice take 40 μl cells and add 1 μl of the dilution

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      Source URL: stockcenter.vdrc.at

      - Date: 2016-02-24 07:59:14
        4MORPHODYNAMICS OF THE SELAWIK RETROGRESSIVE THAW SLUMP, NORTHWEST ALASKA by Theodore B. Barnhart

        MORPHODYNAMICS OF THE SELAWIK RETROGRESSIVE THAW SLUMP, NORTHWEST ALASKA by Theodore B. Barnhart

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        Source URL: geology.isu.edu

        - Date: 2013-06-03 13:06:27
          5Freeze Thaw Damage Potential Concrete products, when exposed to freeze thaw cycles and deicing salts will deteriorate. If you have been inside a parking garage, the effects of winter in Central New York are obvious. Simi

          Freeze Thaw Damage Potential Concrete products, when exposed to freeze thaw cycles and deicing salts will deteriorate. If you have been inside a parking garage, the effects of winter in Central New York are obvious. Simi

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          Source URL: www.barnesandcone.net

          - Date: 2016-05-16 09:22:14
            6

            Using LTPP Data to Develop Spring Load Restrictions The thawing index (TI) is used by State/Provincial Agencies to set their spring load restriction (SLR). It indicates the progression of thaw in the pavement and the beg

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            Source URL: cies.mst.edu

            - Date: 2015-07-09 14:19:43
              7SOP: Collaborative Cross Project Revised: May 13, 2011 1. Sample Preparation and Setup Thaw the mice the night before in the refrigerator. Roughly 6 to 7 mice can be scanned per day (i.e., 6 to 7 hours of scanning).

              SOP: Collaborative Cross Project Revised: May 13, 2011 1. Sample Preparation and Setup Thaw the mice the night before in the refrigerator. Roughly 6 to 7 mice can be scanned per day (i.e., 6 to 7 hours of scanning).

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              Source URL: www.facebase.org

              Language: English - Date: 2016-06-21 18:31:13
              8The Importance of “Deep” Organic Carbon in Permafrost-Affected Soils of Arctic Alaska J. G. Bockheim* Dep. of Soil Science Univ. of Wisconsin

              The Importance of “Deep” Organic Carbon in Permafrost-Affected Soils of Arctic Alaska J. G. Bockheim* Dep. of Soil Science Univ. of Wisconsin

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              Source URL: www.globalcarbonproject.org

              Language: English - Date: 2008-12-02 00:43:34
              9INFLUENCE OF SOIL PROFILE PROPERTIES ON RESPIRED CARBON DIOXIDE (CO2) AND METHANE (CH4) GASES BEFORE, DURING, AND AFTER DISTURBANCE BY A RETROGRESSIVE THAW SLUMP ALONG THE SELAWIK RIVER, NW ALASKA. by

              INFLUENCE OF SOIL PROFILE PROPERTIES ON RESPIRED CARBON DIOXIDE (CO2) AND METHANE (CH4) GASES BEFORE, DURING, AND AFTER DISTURBANCE BY A RETROGRESSIVE THAW SLUMP ALONG THE SELAWIK RIVER, NW ALASKA. by

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              Source URL: geology.isu.edu

              Language: English - Date: 2013-09-30 12:03:07
              10PERMAFROST DEGRADATION AND RIVER METABOLISM: DOWNSTREAM EFFECTS OF RETROGRESSIVE THAW SLUMP SEDIMENTATION ON ECOSYSTEM METABOLISM IN THE SELAWIK RIVER, ALASKA by

              PERMAFROST DEGRADATION AND RIVER METABOLISM: DOWNSTREAM EFFECTS OF RETROGRESSIVE THAW SLUMP SEDIMENTATION ON ECOSYSTEM METABOLISM IN THE SELAWIK RIVER, ALASKA by

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              Source URL: geology.isu.edu

              Language: English - Date: 2012-12-17 12:44:58