Transformation Procedure 1. Take ice, put DH5a, wait to thaw 2. Aliquote 50 ul to prechilled 600 ul tubes 3. Add 1 ul DNA to tubes and wait for 30 min on ice. 4. Heat shock 42oC for 45 sec. 5. Incubate on ice for 2 min

Source URL: wang.eng.ucsd.edu

ORIGINAL RESEARCH ARTICLE published: 11 December 2014 doi: fmicbSpring thaw ionic pulses boost nutrient availability and microbial growth in entombed Antarctic Dry Valley

Source URL: glaciers.pdx.edu

Bacteria transformation spin down → 1 μl add 9 μl H2O thaw chemo-competent E.coli SURE cells (Stratagene) on ice take 40 μl cells and add 1 μl of the dilution

Source URL: stockcenter.vdrc.at

MORPHODYNAMICS OF THE SELAWIK RETROGRESSIVE THAW SLUMP, NORTHWEST ALASKA by Theodore B. Barnhart

Source URL: geology.isu.edu

Freeze Thaw Damage Potential Concrete products, when exposed to freeze thaw cycles and deicing salts will deteriorate. If you have been inside a parking garage, the effects of winter in Central New York are obvious. Simi

Source URL: www.barnesandcone.net

Using LTPP Data to Develop Spring Load Restrictions The thawing index (TI) is used by State/Provincial Agencies to set their spring load restriction (SLR). It indicates the progression of thaw in the pavement and the beg

Source URL: cies.mst.edu

SOP: Collaborative Cross Project Revised: May 13, 2011 1. Sample Preparation and Setup Thaw the mice the night before in the refrigerator. Roughly 6 to 7 mice can be scanned per day (i.e., 6 to 7 hours of scanning).

Source URL: www.facebase.org

• Medical imaging
• Materials science
• Microtechnology
• Tomography
• X-ray microtomography
• Scan
• CT scan
• Mouse button
• Technology
• Chemistry
• Vision

The Importance of “Deep” Organic Carbon in Permafrost-Affected Soils of Arctic Alaska J. G. Bockheim* Dep. of Soil Science Univ. of Wisconsin

Source URL: www.globalcarbonproject.org

• Physical geography
• Permafrost
• Pedology
• Geography
• Soil science
• Active layer
• Gelisol
• Thaw depth
• Soil
• Lake
• Cold Regions Research and Engineering Laboratory
• Periglaciation

INFLUENCE OF SOIL PROFILE PROPERTIES ON RESPIRED CARBON DIOXIDE (CO2) AND METHANE (CH4) GASES BEFORE, DURING, AND AFTER DISTURBANCE BY A RETROGRESSIVE THAW SLUMP ALONG THE SELAWIK RIVER, NW ALASKA. by

Source URL: geology.isu.edu

• Land management
• Pedology
• Soil science
• Carbon
• Soil
• Permafrost

PERMAFROST DEGRADATION AND RIVER METABOLISM: DOWNSTREAM EFFECTS OF RETROGRESSIVE THAW SLUMP SEDIMENTATION ON ECOSYSTEM METABOLISM IN THE SELAWIK RIVER, ALASKA by

Source URL: geology.isu.edu

• Pedology
• Selawik
• Arctic Ocean
• Permafrost
• Ecosystem
• Alaska