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Yeast Two-Hybrid Procedure 1. Generate a GAL4 DNA-BD fusion by cloning the gene of interest in frame with the GAL4 DNA binding domain of pGBKT7. 2. Transform AH109 with bait plasmid, detect bait expression and test for a
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Yeast Two-Hybrid Procedure 1. Generate a GAL4 DNA-BD fusion by cloning the gene of interest in frame with the GAL4 DNA binding domain of pGBKT7. 2. Transform AH109 with bait plasmid, detect bait expression and test for a