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31

Transformation Procedure 1. Take ice, put DH5a, wait to thaw 2. Aliquote 50 ul to prechilled 600 ul tubes 3. Add 1 ul DNA to tubes and wait for 30 min on ice. 4. Heat shock 42oC for 45 sec. 5. Incubate on ice for 2 min

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Source URL: wang.eng.ucsd.edu

Language: English - Date: 2017-09-15 13:58:59
    32

    It was dusk by now and the comisaria was almost empty. A uniformed man with two gold teeth sat at a small wooden table in the lobby; he told me to wait on the bench. A few moments later, nothing having happened, he gestu

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    Source URL: www.arnokopecky.com

    Language: English - Date: 2013-01-05 16:06:41
      33

      Please wait... If this message is not eventually replaced by the proper contents of the document, your PDF viewer may not be able to display this type of document. You can upgrade to the latest version of Adobe Reader fo

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      Source URL: www.alaska.edu

      - Date: 2017-10-26 20:42:00
        34

        Please wait... If this message is not eventually replaced by the proper contents of the document, your PDF viewer may not be able to display this type of document. You can upgrade to the latest version of Adobe Reader fo

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        Source URL: www.uhfoundation.org

        - Date: 2015-12-02 14:05:47
          35

          Wait List Application GARAGE ▪ LOT DOWNTOWN/UNIVERSITY HILL INFORMATION & APPLICATION

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          Source URL: www-static.bouldercolorado.gov

          Language: English - Date: 2018-01-29 17:43:46
            36

            Please wait... If this message is not eventually replaced by the proper contents of the document, your PDF viewer may not be able to display this type of document. You can upgrade to the latest version of Adobe Reader fo

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            Source URL: www.tap-ag.com

            - Date: 2017-12-04 04:59:59
              37

              Please wait... If this message is not eventually replaced by the proper contents of the document, your PDF viewer may not be able to display this type of document. You can upgrade to the latest version of Adobe Reader fo

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              Source URL: www.berliner-sparkasse.de

                38

                October 2017 Judging Results Looks like cooler weather might be settling in for a while. Hold back on your watering if the mornings are chilly. If you feel you need to water, wait until mid-day when it is hopefully, the

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                Source URL: www.orchidseeos.com

                Language: English - Date: 2018-04-18 06:25:51
                  39

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                  Source URL: www.lsbpne.com

                  - Date: 2018-02-15 13:58:00
                    40

                    Please wait... If this message is not eventually replaced by the proper contents of the document, your PDF viewer may not be able to display this type of document. You can upgrade to the latest version of Adobe Reader fo

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                    Source URL: www.abt2-rk.tu-berlin.de

                    - Date: 2016-09-29 06:47:30
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