gRNA Synthesis Protocol STEP 1: Find all 23bp genomic sites of the form 5’-N20NGG-3’ near your intended target site (ideally ±50bp). These may reside on the or – strand. PAM 5’!NNNNN NNNNN NNNNN NNNNN NGG!3’ - DNA synthesis - Document - PDFSEARCH.IO

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	gRNA Synthesis Protocol STEP 1: Find all 23bp genomic sites of the form 5’-N20NGG-3’ near your intended target site (ideally ±50bp). These may reside on the + or – strand. PAM 5’!NNNNN NNNNN NNNNN NNNNN NGG!3’ Add to Reading List Document Date: 2014-11-05 23:32:09 Open Document File Size: 115,20 KB Share Result on Facebook Company IDT / / Country Mali / / Facility UUUU gRNA scaffold AGCCACG GUGAAAAAGUUC / RNA scaffold / / IndustryTerm intended target site / / Organization Church Lab / / Product Gibson / / Technology http / cloning / human genome / pdf / / URL http / SocialTag Chemistry Guide RNA Polymerase chain reaction Oligonucleotide Molecular cloning Vector DNA microarray Gene expression Biology Molecular biology

gRNA Synthesis Protocol STEP 1: Find all 23bp genomic sites of the form 5’-N20NGG-3’ near your intended target site (ideally ±50bp). These may reside on the + or – strand. PAM 5’!NNNNN NNNNN NNNNN NNNNN NGG!3’Add to Reading List