pAC161-SeqFinal page < PAGE >1 of < NUMPAGES >4 Plasmid name: pAC161 Remarks: complete sequence, reanalysed on both strands T-DNA is marked in yellow (compare map) EcoRI site in map is numbered "1" in sequence

Source URL: www.gabi-kat.de

Produce E. coli gcvR Promoter with Oligos The goal is to have the workshop make this new promoter with BioBrick ends. Will use oligo assembly method. Desired sequence: Need biobrick prefix end with EcoRI sticky end -35

Source URL: bio.davidson.edu

EcoRI (396bp) VCre pUCNLS-VCre (3911bp)

Source URL: www.biosupport.kazusa.or.jp

EcoRI (396bp) SacI (406bp) KpnI (412bp) VloxP MluI (482bp) NotI (489bp)

Source URL: www.biosupport.kazusa.or.jp

DNA purification with SigmaSpin post-reaction cleanup-plates required: centrifuge capable of spinning 96 well plates (we used a Heraeus 3SR) dont forget: after reaction, before cleanup: heat-inactivate encyme: e.g. EcoRI

Source URL: stockcenter.vdrc.at

Microsoft Word - ISI_str_07.doc

Source URL: www.ibt.lt

• Biotechnology
• Biology
• Molecular biology
• Nature
• Restriction enzymes
• Endonuclease
• Nuclease
• DNA
• EcoRI
• Virus

pAC161-SeqFinal page < PAGE >1 of < NUMPAGES >4 Plasmid name: pAC161 Remarks: complete sequence, reanalysed on both strands T-DNA is marked in yellow (compare map) EcoRI site in map is numbered "1" in sequence

Source URL: www.gabi-kat.de

CLONING INVERTED REPEATS IN HIGH THROUGHPUT This protocol is calculated for cloning one 96 well plate 1. design primers: as standard we include a EcoRI restriction site on the 5’ primer and XbaI on the 3’ primer; the

Source URL: stockcenter.vdrc.at

• Molecular biology
• Polymerase chain reaction
• Ligation
• Agar
• Primer
• Gel extraction
• Cloning vector
• Molecular cloning

- Front Page Journal http://www.ecori.org/front-page-journalnew-city-gard... New City Garden Provides Food and Jobs Monday, July 9, 2012 at 6:57PM

Source URL: www.thesteelyard.org

PDF Document

Source URL: phagesdb.org

• Biochemistry
• Restriction enzyme
• Restriction digest
• Polymerase chain reaction
• DNA ladder
• Ethidium bromide
• Agar
• EcoRI
• HaeIII
• Biology
• Molecular biology
• Chemistry