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City Beverly / Boston / Poisson / Eppendorf / Cambridge / / Company GenBank / Millipore / Iron Mountain / Agencourt Biosciences / Amersham / Affymetrix / Qiagen / IDT / Pearson / Genaissance Pharmaceuticals / Hess / Genomic Solutions / Wolter L.C. / / Country Bermuda / United States / / Currency cent / STD / / / Event FDA Phase / Product Issues / / Facility Harvard Medical School Biopolymers Facility / Biopolymer Core Facility / Station ALOHA / DOE Joint Genome Institute / Energy Joint Genome Institute / US Department of Energy Joint Genome Institute / Massachusetts Institute of Technology / / IndustryTerm experimental protocol / iterative genome assembly algorithm / homemade shearing device / enzymatic processing methods / blunt-end cloning protocol / assembly algorithms / The ligation product / strict sample-handling protocol / ml salt solution / library-construction protocol / ligation protocol / strand-displacement amplification applications / real-time amplification method / library construction protocol / OnLine Database / alkaline solution / / MedicalCondition single tumor / inherited disease / / NaturalFeature Gulf Stream / Sargasso Sea / Pacific Ocean / / Organization Harvard Medical School / US Department of Energy / National Science Foundation / Department of Energy Joint Genome Institute / Harvard / Cummings Center / Massachusetts Institute of Technology / Moore Foundation / Genomes-to-Life Center / US Federal Reserve / DOE Joint Genome Institute / / Person Nat / Stratagene / / / Position Dean / AUTHOR / model / / Product S1 / bp 100 / Nokia E71 Smartphone / sodium acetate / HotStar PCR MasterMix (Qiagen) / MIT9312 / B10 / l-phage / / ProgrammingLanguage Perl / / ProvinceOrState California / Massachusetts / / PublishedMedium NATURE BIOTECHNOLOGY / / Technology Genomics / Adam / Bioinformatics / electrophoresis / iterative genome assembly algorithm / sequencing library construction protocol / hybridization / BIOTECHNOLOGY / two-step ligation protocol / cloning / Perl / 684 assembly algorithms / html / thermocycling protocol / DOE-JGI sequencing library construction protocol / human genome / E. coli Antisense Genome chips / strict sample-handling protocol / library-construction protocol / MDA protocol / E. coli Antisense Genome chip / electroporation / one-step ligation protocol / regular MDA protocol / DNA sequencing / Environmental Engineering / recombination system / DNA sequencing technologies / cloning system / http / library construction protocol / DNA amplification / experimental protocol / same protocol / gel electrophoresis / Invitrogen TOPO blunt-end cloning protocol / using the same protocol / / URL http / SocialTag 
© 2006 Nature Publishing Group http://www.nature.com/naturebiotechnology ARTICLES Sequencing genomes from single cells by polymerase cloning