| Document Date: 2013-09-20 10:13:14
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City Eppendorf / / Company QIAgen / Beckman Coulter / IgG / 1mM dATP / / Facility Library Quantification / Library Purification Use Agencourt Ampure / Library Construction Protocol Preparation / Capture antibody/chromatin complex / / IndustryTerm formaldehyde solution / discard solution / media containing formaldehyde / culture media / inhibitor stock solutions / stock solution / library protocol / chemical hood / collection media / input energy / / Position Forward / / Product sterile water / / Technology 352 H3K4me1 Diagenode pAb-037-050 H3K4me3 H3K36me3 Cell Signaling Technology Cell Signaling Technology Diagenode* Cell Signaling Technology / Cell Signaling Technology / 50 H3me3K27 Cell Signaling Technology / 6 REMC Library Construction Protocol The library protocol / 80 µl ChIP / following PCR protocol / 34 µl ChIP / 12 ChIP / Antibody name H3K9Ac Antibody Provider Cell Signaling Technology / control ChIP / flash / antibodies / 4 volumes ChIP / /
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Farnham Lab_REMC ChIP and Library Construction Protocol (Oct29_2010)