IP of AMPK for kinase assay After transfection of cells is complete: Remove media from plate Add 1ml lysis buffer to plate. Scrape cells and transfer to a fuge tube. Keep on ice for 10 min. Spin for 5 min @ 14000g @

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		IP of AMPK for kinase assay After transfection of cells is complete: Remove media from plate Add 1ml lysis buffer to plate. Scrape cells and transfer to a fuge tube. Keep on ice for 10 min. Spin for 5 min @ 14000g @ Add to Reading List Source URL: www.med.upenn.edu Download Document from Source Website File Size: 11,50 KB Share Document on Facebook