IP of AMPK for kinase assay After transfection of cells is complete: Remove media from plate Add 1ml lysis buffer to plate. Scrape cells and transfer to a fuge tube. Keep on ice for 10 min. Spin for 5 min @ 14000g @ - Spin ice - Document - PDFSEARCH.IO

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	IP of AMPK for kinase assay After transfection of cells is complete: Remove media from plate Add 1ml lysis buffer to plate. Scrape cells and transfer to a fuge tube. Keep on ice for 10 min. Spin for 5 min @ 14000g @ Add to Reading List Open Document File Size: 11,50 KB Share Result on Facebook

IP of AMPK for kinase assay After transfection of cells is complete: Remove media from plate Add 1ml lysis buffer to plate. Scrape cells and transfer to a fuge tube. Keep on ice for 10 min. Spin for 5 min @ 14000g @Add to Reading List