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Contig / 454 Life Sciences / Shotgun sequencing / Bacteriophage / Genomics / Full genome sequencing / Lambda phage / Sequencing / Genome project / Biology / Molecular biology / DNA sequencing


Analysis of High-Throughput Sequencing and Annotation Strategies for Phage Genomes Matthew R. Henn1*, Matthew B. Sullivan2¤a, Nicole Stange-Thomann1, Marcia S. Osburne2, Aaron M. Berlin1, Libusha Kelly2, Chandri Yandava
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Document Date: 2010-07-28 19:18:16

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City

Tucson / Universidad Miguel Hernandez / Branford / Eppendorf / Cambridge / Foster City / /

Company

Genome Size (bp) No. ORFs GenBank / Cambrex Bio Science Rockland Inc. / Genbank / Agilent Technologies / Millipore / Fisher Scientific / blastLoci Stop Start Only Broad Only GenBank / QIAGEN / Agencourt Bioscience Corporation / Creative Commons / Covaris Inc. / /

Country

United States / /

Currency

pence / USD / /

/

Event

FDA Phase / Man-Made Disaster / /

Facility

Linker Amplified Shotgun Library / Library ID Nuclease / AA pipeline / University of Arizona / P-SSP7 WGSL library / Broad Institute / Massachusetts Institute of Technology / Broad Institute of MIT / /

IndustryTerm

homology search / assembly algorithm / library construction protocols / assembly software packages / gel electrophoresis equipment / non-contact controlled isothermal mechanical energy / ligation products / assembly software / marine systems / diluted bleach solution / flow cell technology / final product / purified ligation products / gene product / annotation infrastructure / library construction protocol / qPCR software / linker solutions / /

Organization

Betty Moore Foundation / DOE / National Science Foundation / MIT / Harvard / Department of Ecology and Evolutionary Biology / Massachusetts Institute of Technology / Broad Institute / Pittsburgh PA / Department of Civil and Environmental Engineering / Raleigh NC / University of Arizona / /

Person

Francisco Rodriguez-Valera / Miguel Hernandez / Gene Model / /

Position

original author / Editor / sequence editor / Author / M13 forward / calibrator / /

Product

S2 Adaptive Focused Acoustics / polyethylene glycol / Newbler software / Pico 6000 chip / acetic acid / Agilent Pico 6000 / Newbler / GS20 / /

ProvinceOrState

Connecticut / Arizona / Massachusetts / /

PublishedMedium

PLoS ONE / the PLoS ONE / /

RadioStation

Berlin AM / /

Technology

genomics / 6000 chip / electrophoresis / gene mapping / FLX protocol / AFA technology / cloning / Newbler assembly algorithm / assembly algorithm / flow cell technology / WGSL protocol / WGSL library construction protocol / electroporation / genetic code / library construction protocols / 1000 chip / Environmental Engineering / 600 Chip / GS20 protocol / LASL protocol / gel electrophoresis / dialysis / /

URL

www.plosone.org / http /

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